FW11-9-2细胞
细胞形态： **样
**类型： IgG2a
运输方式： 冻存运输
数量： 大量
生长状态： 悬浮生长
ATCC Number： HB-256™
是否是肿瘤细胞： 0
物种来源： 小鼠
Designations: FW11-9-2
Depositors: CR Mackay
Isotype: IgG2a
FW11-9-2细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
FW11-9-2细胞Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against the v6 variant of human CD44
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: FW11-9-2细胞Animals were immunized with a fusion protein corresponding to the variant region of human CD44 (exons 3v to 10v).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for the product of the 6v exon.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22355: Mackay CR, et al. FW11-9-2细胞Expression and modulation of CD44 variant isoforms in humans. J. Cell Biol. 124: 71-82, 1994. PubMed: 7507492
