Toledo细胞
运输方式： 冻存运输
数量： 大量
ATCC Number： CRL-2631™
相关**： 其他**
生长状态： 悬浮生长
年限： *****
器官来源： 外周血
细胞类型： B**细胞
是否是肿瘤细胞： 0
物种来源： 人
细胞形态： **样
Designations: Toledo
Toledo细胞Depositors: H Ben-Bassat
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens deposited as human
Morphology: lymphoblast
Source: Organ: peripheral blood
Disease: diffuse large cell lymphoma; non-Hodgkin's B cell lymphoma
Cell Type: B lymphocyte;
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Isolation: Isolation date: 1990
Antigen Expression: CD10 +, CD19 +, CD20 +, CD38 +, CD23 -, CD39 - [53289]
DNA Profile (STR): D5S818:10,12
D7S820: 9,10
THO1: 8,9.3
TPOX: 8,11
Toledo细胞vWA: 16,17
Amelogenin: X
CSF1PO: 12
D13S317:11
D16S539:9
Age: *****
Gender: female
Ethnicity: White
Comments: Toledo was established in 1990 from peripheral blood leukocytes (PBL) of a patient that originally had a diffuse large cell lymphoma (DLCL). [53289]
Following high-dose chemotherapy and bone marrow transplantation, the patient subsequently developed a lymphoma in the brain. [53289]
Though the morphology of the cells resembles Burkitt's lymphoma, the cells lack the typical chromosomal translocations of Burkitt's lymphoma. The karyotype does exhibit multiple chromosomal aberrations. [53289]
The cells do not express surface or cytoplasmic immunoglobulin. [53289]
The cells are negative for Epstein-Barr virus. [53289]
This cell line is a model system for studying non-Hodgkin lymphomas.
Propagation: Toledo细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 X 10(5) viable cells/ml. Maintain cultures at cell concentrations between 3 X 10(5) and 3 X 10(6) viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 24 to 30 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 53289: Gabay C, et al. Toledo细胞Somatic mutations and intraclonal variations in the rearranged Vkappa genes of B-non-Hodgkin's lymphoma cell lines. Eur. J. Immunol. 63: 180-191, 1999. PubMed: 10485273
