HCC1954 BL细胞
运输方式： 冻存运输
数量： 大量
细胞形态： **样
生长状态： 悬浮生长，多细胞聚集
年限： 61 years *****
细胞类型： B**细胞
ATCC Number： CRL-2339™
器官来源： 外周血
是否是肿瘤细胞： 0
物种来源： 人
HCC1954 BL细胞Designations: HCC1954 BL
Depositors: AF Gazdar, AK Virmani
Biosafety Level: 2 [Cells contain Herpesvirus; Epstein Barr Virus (EBV) ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension, multicell aggregates
Organism: Homo sapiens
Morphology: lymphoblast
Source: Organ: peripheral blood
Cell Type: B lymphoblast; Epstein-Barr virus (EBV) transformed
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: HCC1954 BL细胞The line is available with the following restrictions:
1. This cell line was deposited at the ATCC by Dr. Adi F. Gazdar and is provided for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purposes of sale, or producing for sale, cells or their products. The cells are provided as service to the research community. They are provided without warranty of merchantability or fitness for a particular purpose or any other warranty, expressed or implied.
2. Any proposed commercial use of the these cells, or their products must first be negotiated with the University of Texas Southwestern Medical Center at Dallas, 5323 Harry Hines Blvd., Dallas, Texas 75235. Telephone (214) 648-1888, Email TechnologyDevelopment@UTSouthwestern.edu, or Fax: (214) 951-0935.
Applications: The cell line was derived from a 61 year-old East Indian female with stage IIA, grade 3, primary ductal carcinoma of the breast.
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,11
D13S317: 8,9
D16S539: 9,11
D5S818: 11,12
D7S820: 10,11
THO1: 6,7
TPOX: 8,9
vWA: 18,19
Age: 61 years *****
Gender: female
Ethnicity: East Indian
Comments: HCC1954 BL is a B lymphoblastoid cell line initiated from peripheral blood lymphocytes from the same patient as HCC1954 (ductal carcinoma of the breast) by transformation with Epstein-Barr virus (EBV).
The cell line was derived from a 61 year-old East Indian female with stage IIA, grade 3, primary ductal carcinoma of the breast.
Propagation: HCC1954 BL细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 x 10 exp5 viable cells/ml. Maintain cultures at cell concentrations between 5 X 10 exp5 and 2 X 10 exp6 viable cells/ml.
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
tumor cell line established from the same patient:ATCC CRL-2338
purified DNA:ATCC CRL-2339D
purified RNA:ATCC CRL-2339R
References: 38266: Gazdar AF, et al. HCC1954 BL细胞Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771
