Reh细胞
数量： 大量
细胞形态： **样
是否是肿瘤细胞： 0
物种来源： 人
ATCC Number： CRL-8286™
相关**： 其他**
运输方式： 冻存运输
生长状态： 悬浮生长
Designations: Reh
Depositors: Univ. Pennsylvania
Biosafety Level: 1
Reh细胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Disease: acute lymphocytic leukemia (non-T; non-B)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: Reh细胞CD3 A (17%) B (17%) C (20%), CD4 (15%), CD10 (55%)
DNA Profile (STR): D5S818: 11, 13
D13S317: 11, 13
D7S820: 9, 12
D16S539: 9
vWA: 14, 15
THO1: 7
TPOX: 8
CSF1PO: 10, 11, 12
Amelogenin: X
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Reh细胞Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: 95% (v/v) FBS; 5% (v/v) DMSO
Storage temperature: Liquid nitrogen vapor phase
References: 22700: Greaves M, Janossy G. Patterns of gene expression and the cellular origins of human leukaemias. Biochim. Biophys. Acta 516: 193-230, 1978. Reh细胞PubMed: 365240
