HGF-1细胞
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 人
年限： 28 years
ATCC Number： CRL-2014™
相关**： 正常
组织来源： gingiva
细胞形态： 成纤维样
生长状态： 贴壁生长
数量： 大量
Designations: HGF-1
Depositors: BS McAllister
Biosafety Level: 1
Shipped: frozen
HGF-1细胞Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Tissue: gingiva
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: October, 1989
Applications: HGF-1细胞Bradykin receptors are present on the cells at an apparent density of 22000 per cells and dissociation constant (Kd) of 1.2 nM. The receptors are coupled to a signaling pathway involving phospholipase C and A2 and calcium channel effectors.
Epidermal growth factor (EGF) and platelet derived growth factor (PDGF) stimulate DNA synthesis and proliferation in HGF-1 cells.
Receptors: bradykinin, expressed
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,12
D16S539: 11,13
D5S818: 12
D7S820: 10
THO1: 6,7
TPOX: 8,11
vWA: 17,18
Age: 28 years
Gender: male
Ethnicity: Caucasian
HGF-1细胞Comments: Bradykin receptors are present on the cells at an apparent density of 22000 per cells and dissociation constant (Kd) of 1.2 nM. The receptors are coupled to a signaling pathway involving phospholipase C and A2 and calcium channel effectors.
Epidermal growth factor (EGF) and platelet derived growth factor (PDGF) stimulate DNA synthesis and proliferation in HGF-1 cells. Bradykinin treatment blocks the proliferation stimulation by EGF and PDGF.
The cells were deposited to ATCC at an unknown passage level. A longevity study performed by ATCC showed that the depositor?s material was capable of growing for an additional 22 population doublings (i.e., unknown + 22) before the onset of senescence.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: HGF-1细胞liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 23126: McAllister BS, et al. Bradykinin receptors and signal transduction pathways in human fibroblasts: integral role for extracellular calcium. Arch. Biochem. Biophys. 304: 294-301, 1993. PubMed: 7686736
23527: McAllister BS, et al. Bradykinin inhibition of EGF- and PDGF-induced DNA synthesis in human fibroblasts. Am. J. Physiol. 265: C477-C484, 1993. PubMed: 8396328
