BNL CL.2细胞
器官来源： 肝
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 小鼠
数量： 大量
生长状态： 贴壁生长
品系： BALB/c
年限： embryo
ATCC Number： TIB-73™
相关**： 正常
Designations: BNL CL.2
Depositors: P Patek, J Collins, M Cohn
BNL CL.2细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology:
Source: Organ: liver
Strain: BALB/c
Disease: normal
BNL CL.2细胞Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: No
Age: embryo
Comments: The line was selected to grow in medium containing ornithine and phenylalanine in place of arginine and tyrosine.
Tested and found negative for ectromelia virus (mousepox).
Propagation: BNL CL.2细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin for 1 minute, remove trypsin and let the culture sit at room temperature until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Related Products: derivative:ATCC TIB-74
References: 22905: Patek PQ, et al. BNL CL.2细胞Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934
