Schneider's Drosophila Line 2 [D. Mel. (2), SL2]细胞
器官来源： 胚胎
年限： embryo; 20 to 24 hours
数量： 大量
生长状态： 悬浮生长
运输方式： 冻存运输
ATCC Number： CRL-1963™
是否是肿瘤细胞： 0
物种来源： 果蝇
细胞形态： 上皮样
Designations: Schneider's Drosophila Line 2 [D. Mel. (2), SL2]
Schneider's Drosophila Line 2 [D. Mel. (2), SL2]细胞Depositors: IR Schneider
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension or loosely adherent
Organism: Drosophila melanogaster
Morphology: epithelial
Source: Organ: embryo
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Isolation: Isolation date: 1969
Applications: transfection host (Roche Transfection Reagents)
Age: embryo; 20 to 24 hours
Comments: Schneider's Drosophila Line 2 [D. Mel. (2), SL2]细胞The cells have been shown to support the growth of the insect stages of malaria parasites [Pubmed ID: 1734521].
Propagation: ATCC complete growth medium: Schneider's Drosophila Medium, 90%; heat-inactivated fetal bovine serum, 10%. NOTE: the fetal bovine serum should be selected for ability to support the growth of insect cell lines.
This medium is formulated for use without CO2.
Temperature: 23.0°C; (Max. 24.0 °C, Min. 22.0°C)
Note: The cells will grow as a loose monolayer (or in suspension) at any temperature between 16.0°C and 28.0°C.
Subculturing: Protocol: Gently resuspend the cells by pipetting medium across the monolayer or (for larger flasks) by slapping the flask against the base of the palm. Schneider's Drosophila Line 2 [D. Mel. (2), SL2]细胞Dilute the cell suspension to the desired concentration with fresh medium.
Maintain cultures at a cell concentration between 5 X 10(4) and 4 X 10(5) cells/cm2.
Subcultivation Ratio: A subcultivation ratio of 1:10 or greater is recommended
Medium Renewal: At the time of subcultivation
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: approximately 67 hours
References: 22960: Warburg A, Miller LH. Schneider's Drosophila Line 2 [D. Mel. (2), SL2]细胞Sporogonic development of a malaria parasite in vitro. Science 255: 448-450, 1992. PubMed: 1734521
22983: Schneider I. Cell lines derived from late embryonic stages of Drosophila melanogaster. J. Embryol. Exp. Morphol. 27: 353-365, 1972. PubMed: 4625067
