ACT IV细胞
ATCC Number： HB-81™
细胞形态： **样
**类型： IgG1
运输方式： 冻存运输
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 悬浮生长
Designations: ACT IV
Depositors: P Parham
Isotype: IgG1
ACT IV细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: ACT IV细胞immunoglobulin; monoclonal antibody; against actin
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Comments: Animals were immunized with actin from Dictyostelium discoideum.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with actin from prokaryotic organisms at an epitope distinct from that recognized by ACT I (ATCC HB-80).
The antibody does not cross-react with eukaryotic actins.
ACT IV细胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.1 mM hypoxanthine, 400 nM aminopterin and 0.016 mM thymidine, 80%; fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2001
Recommended serum: ATCC 30-2020
HAT (Hypoxanthine-Aminopterin-Thymidine): ATCC 69-X
References: 986: Simpson PA, et al. ACT IV细胞Monoclonal antibodies prepared against Dictyostelium actin: characterization and interactions with actin. J. Cell Biol. 99: 287-295, 1984. PubMed: 6203918
