SVR bag4细胞
数量： 大量
生长状态： 贴壁生长
运输方式： 冻存运输
组织来源： islet of Langerhans; endothelium
ATCC Number： CRL-2387™
细胞类型： SV40转化细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
器官来源： 胰腺
Designations: SVR bag4
Depositors: JL Arbiser
SVR bag4细胞Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus deposited as mouse
Morphology:
Source: Organ: pancreas
Tissue: islet of Langerhans; endothelium
Cell Type: endothelialSV40 transformed
Permits/Forms: SVR bag4细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: SVR bag4 is an endothelial cell line derived from the SVR cell line (ATCC CRL-2280) by infecting SVR cells with a retrovirus encoding beta-galactosidase and puromycin resistance.
SVR is a hygromycin resistant mouse endothelial cell line containing a temperature sensitive SV40 large T-antigen and H-RAS oncogene.
SVR bag4 can be used as a wild-type control for SVR A221a (ATCC CRL-2386). SVR bag4细胞It can be used to determine if drugs regulate the MAPKK pathway.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 1.0 g/L glucose, 95%; fetal bovine serum, 5%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 51400: LaMontagne KR Jr., et al. SVR bag4细胞Inhibition of MAP kinase kinase causes morphological reversion and dissociation between soft agar growth and in vivo tumorigenesis in angiosarcoma cells. Am. J. Pathol. 157: 1937-1945, 2000. PubMed: 11106566
