GPC-16细胞
生长状态： 贴壁生长
细胞形态： 上皮样
器官来源： 结肠
ATCC Number： CCL-242™
相关**： 大肠癌
数量： 大量
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 豚鼠
Designations: GPC-16
Depositors: RW O'Donnell
GPC-16细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Cavia porcellus
Morphology: epithelial
Source: Organ: colon
Disease: colorectal adenocarcinoma
Cellular Products: keratin
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Tumorigenic: Yes
Comments: PAS, positive.
The cells are positive for keratin by immunoperoxidase staining.
Propagation: GPC-16细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 23041: O'Donnell RW, Cockerell GL. GPC-16细胞Establishment and biological properties of a guinea pig colonic adenocarcinoma cell line induced by N-methyl-N-nitrosourea. Cancer Res. 41: 2372-2377, 1981. PubMed: 7237435
