N418细胞
细胞形态： **样
**类型： IgG
数量： 大量
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 仓鼠
生长状态： 悬浮生长
ATCC Number： HB-224™
Designations: N418
Depositors: RM Steinman
Isotype: IgG
Biosafety Level: 1
N418细胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma) deposited as hamster, Armenian (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; N418细胞against mouse leukocyte integrin (CD11c)
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Comments: Animals were immunized with mouse dendritic cells.
Spleen cells were fused with Sp2/0 myeloma cells.
The antibody reacts precipitates a 150000/90000 dalton CD11/CD18 heterodimer (see also ATCC HB-226) from murine dendritic cells.
The N418 epitope may be the mouse equivalent to human CD11c.
The N418 epitope is detected only on dendritic cells and is not present on peritoneal macrophages, anti-immunoglobulin induced B lymphoblasts or bulk lymph node cells.
Propagation: N418细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 1793: Metlay JP, et al. N418细胞The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J. Exp. Med. 171: 1753-1771, 1990. PubMed: 2185332
