MDBK (NBL-1)细胞
生长状态： 贴壁生长
数量： 大量
细胞形态： 上皮样
ATCC Number： CCL-22™
相关**： 正常
年限： *****
是否是肿瘤细胞： 0
物种来源： 牛
运输方式： 冻存运输
器官来源： 肾脏
Designations: MDBK (NBL-1)
MDBK (NBL-1)细胞Depositors: S Madin, NB Darby
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Bos taurus
Morphology: epithelial
Source: Organ: kidney
Disease: normal
Cellular Products: keratin
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Applications: MDBK (NBL-1)细胞detection and quantitation of replication competent retroviruses [92434]
transfection host
Virus Resistance: poliovirus 2
Cytogenetic Analysis: Chromosome Frequency Distribution 50 Cells: 2n = 60. The stemline chromosome number is hypodiploid with 2S component occurring at 5%., There were a total of 11-14 marker chromosomes (4-5 metacentric, 3-4 submetacentric and 4-5 acro-telocentric) common to most hypodiploid metaphases. The X was monosomic. Neither HSR chromosomes nor DM's were seen.
Age: *****
Gender: male
Comments: The MDBK cell line was derived from a kidney of an apparently normal ***** steer, February 18, 1957, by S.H. Madin and N.B. Darby.
It was ascertained in 1973 that the MDBK line was infected with bovine viral diarrhea virus (BVDV).
In 1982 R.A. Van Deusen of the NVSL, USDA, Ames, Iowa informed the ATCC that they had found an MDBK free of BVDV infection.
The cells were originally obtained in July, 1967 at passage 96 from the ATCC as a frozen ampule (F-145). Extensive testing (35 passages) by the NVSL found no evidence of any viral contamination in this particular progeny of MDBK.
The cell line was submitted to the American Type Culture Collection by R.A. Van Deusen in passage 110 in August, 1982.
The cells are positive for keratin by immunofluorescence.
MDBK (NBL-1)细胞The line has been shown to be free of bovine viral diarrhea virus (BVDV). [22883]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Rinse the cell sheet twice with fresh 0.25% trypsin, 0.03% EDTA solution, and remove the trypsin solution. Allow the flask to stand at room temperature for 10 to 15 minutes, add fresh medium, aspirate and dispense into new flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
References: 22217: Madin SH, Darby NB Jr.. Established kidney cell lines of normal ***** bovine and ovine origin. Proc. Soc. Exp. Biol. Med. 98: 574-576, 1958. PubMed: 13567776
22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321
32728: Russell DW, Miller AD. MDBK (NBL-1)细胞Foamy virus vectors. J. Virol. 70: 217-222, 1996. PubMed: 8523528
92434: USEPA Manual of Methods for Virology - EPA publication. Washington, DC:Environmental Protection Agency;EPA EPA 600/4-84/013 (R9), 2001
