MDA-MB-134-VI细胞
是否是肿瘤细胞： 0
物种来源： 人
ATCC Number： HTB-23™
相关**： 导管癌
组织来源： duct
数量： 大量
年限： 47 years *****
细胞形态： 上皮样
生长状态： 单层生长
器官来源： **
运输方式： 冻存运输
Designations: MDA-MB-134-VI
Depositors: R Cailleau
MDA-MB-134-VI细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: monolayer (lightly adherent)
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: mammary gland; breast
Tissue: duct
Disease: ductal carcinoma
Derived from metastatic site: pleural effusion
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Receptors: fibroblast growth factor (FGF)
Antigen Expression: Blood Type O; Rh+
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,12
D13S317: 8
D16S539: 13
D5S818: 11,12
D7S820: 10,11
THO1: 6,9
MDA-MB-134-VI细胞TPOX: 8,11
vWA: 14,15
Cytogenetic Analysis: hypodiploid; modal number = 43 with an extra long chromosome 2-like marker; (P28/29) hypodiploid and hypertriploid to hypotetraploid with large subtelocentric marker
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 1
PGM1, 1
PGM3, 1
Age: 47 years *****
Gender: female
Ethnicity: Caucasian
Comments: This is one of a series of breast tumor lines (ATCC HTB-23 through 26) isolated from pleural effusions and characterized by R. Cailleau and associates in 1973.
The cells grow a monolayer of round loosely adherent cells.
They grow slowly, becoming heavy (shedding somewhat into the medium) without actually becoming confluent.
The cells overexpress FGF receptors, and the fgfr-1 gene is amplified.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Atmosphere: air, 100%
Temperature: 37.0°C
Subculturing: Protocol: MDA-MB-134-VI细胞Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: lquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008
recommended serum:ATCC 30-2020
References: 1206: Brinkley BR, et al. Variations in cell form and cytoskeleton in human breast carcinoma cells in vitro. Cancer Res. 40: 3118-3129, 1980. PubMed: 7000337
22182: Cruciger Q, et al. Morphological, biochemical and chromosomal characterization of breast tumor lines from pleural effusions. In Vitro 12: 331, 1976.
22429: Siciliano MJ, et al. Mutually exclusive genetic signatures of human breast tumor cell lines with a common chromosomal marker. Cancer Res. 39: 919-922, 1979. PubMed: 427779
22442: McLeskey SW, et al. MDA-MB-134 breast carcinoma cells overexpress fibroblast growth factor (FGF) receptors and are growth-inhibited by FGF ligands. Cancer Res. 54: 523-530, 1994. PubMed: 7506125
22532: Cailleau R, et al. Breast tumor cell lines from pleural effusions. J. Natl. Cancer Inst. 53: 661-674, 1974. PubMed: 4412247
22536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
22656: Cailleau R, et al. Long-term human breast carcinoma cell lines of metastatic origin: preliminary characterization. In Vitro 14: 911-915, 1978. PubMed: 730202
23093: Faust JB, Meeker TC. MDA-MB-134-VI细胞Amplification and expression of the bcl-1 gene in human solid tumor cell lines. Cancer Res. 52: 2460-2463, 1992. PubMed: 1568216
26319: Cruciger QV, et al. Human breast carcinomas: marker chromosomes involving 1q in seven cases. Cytogenet. Cell Genet. 17: 231-235, 1976. PubMed: 1001030
