PC-12 Adh细胞
细胞形态： 多边形
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 大鼠
生长状态： 贴壁生长
数量： 大量
ATCC Number： CRL-1721.1™
相关**： 其他**
器官来源： 肾上腺
Designations: PC-12 Adh
Depositors: B Patterson
PC-12 Adh细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent, small clusters
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: polygonal
Source: Organ: adrenal gland
Disease: pheochromocytoma
Cellular Products: catecholamines; dopamine; norepinephrine [1163]
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Applications: transfection host
Tumorigenic: Yes
Cytogenetic Analysis: 40 chromosomes; 38 autosomes plus XY [1163]
Gender: PC-12 Adh细胞male
Comments: The PC-12 cell line was derived from a transplantable rat pheochromocytoma. The cells do not synthesize epinephrine. This adherent variant (PC-12 Adh) has been adapted to Corning CellBIND® flasks to improve cell attachment.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Volumes used for this protocol are for a 75cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Remove and discard old culture medium.
Pipet 10 ml fresh medium over the cell sheet and scrape.
Aspirate cells with a small bore pipette to break up clusters.
Add appropriate aliquots of the cell suspension to new Corning CellBIND® 75 cm2 flask with 15 ml fresh growth medium. Seed flask at 1.0 x 10(4) to 3.0x 10(4) viable cells / cm2 or use subcultivation ratio of 1:3 twice weekly.Subculture when cell density reaches between 1.0x 1 0(5) to 2.0x 10(5) viable cells / cm2.
Place culture vessels in incubator at 37�C.
Medium Renewal: Every 2 to 3 days
Preservation:PC-12 Adh细胞Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 48 hrs
Related Products: Parent cell line: CRL-1721, PC-12
Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2004
recommended serum: ATCC 30-2020
recommended serum: ATCC 30-2040
References: 1162: Levi A, et al. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed: 3839317
1163: Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897
22344: Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712
33014: Weber E, et al. PC-12 Adh细胞Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125
