4H1-A7细胞
运输方式： 冻存运输
ATCC Number： CRL-1813™
数量： 大量
生长状态： 悬浮生长
细胞形态： **样
是否是肿瘤细胞： 0
物种来源： 小鼠
**类型： IgG2a
Designations: 4H1-A7
Depositors: CA Whetstone
4H1-A7细胞Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; 4H1-A7细胞against canine adenovirus type 2 (CAV-2)
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Tumorigenic: 4H1-A7细胞Yes
Comments: Animals were immunized with purified CAV-2 (Manhattan strain).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody binds to and neutralizes CAV-2 and is type specific.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 92.5%; horse serum, 5%; fetal bovine serum, 2.5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 4H1-A7细胞22210: Whetstone CA. Monoclonal antibodies to canine adenoviruses 1 and 2 that are type- specific by virus neutralization and immunofluorescence. Vet. Microbiol. 16: 1-8, 1988. PubMed: 3281371
23146: Whetstone CA. Should the criteria for species distinction in adenoviruses be reconsidered? Evidence from canine adenoviruses 1 and 2. Intervirology 23: 116-120, 1985. PubMed: 3884549
