He We细胞
生长状态： 贴壁生长
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 人
数量： 大量
器官来源： 皮肤
ATCC Number： CRL-1338™
相关**： 其他**
细胞形态： 成纤维样
Designations: He We
He We细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: Stiff Skin syndrome
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. He We细胞Please click here for information regarding the specific requirements for shipment to your location.
Gender: male
Comments: He We细胞The tissue donor suffered from Stiff Skin syndrome.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. He We细胞Subculture every 8 to 10 days.
