91MS441细胞
生长状态： 悬浮生长
细胞形态： **样
**类型： IgG1; kappa light chain
运输方式： 冻存运输
数量： 大量
是否是肿瘤细胞： 0
物种来源： 小鼠
ATCC Number： HB-11301™
Designations: 91MS441
Depositors: University of Massachusetts
Isotype: IgG1; kappa light chain
91MS441细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
91MS441细胞Cellular Products: immunoglobulin; monoclonal antibody; against an antibody to a chlamydia genus antigen (anti-idiotypic antibody)
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Comments: Animals were immunized with 89MS30 (GLXA-Ab1) IgG.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
Propagation: 91MS441细胞ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and RPMI 1640 medium with 3 mM L-glutamine and 4.5 g/L glucose, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.
Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
Preservation: culture medium 95%; DMSO, 5%
Related Products: recommended serum:ATCC 30-2020
References: 40475: MacDonald B, et al. 91MS441细胞Oral vaccine comprising anti-idiotypic antibody to chlamydia glycolipid exoantigen and process. US Patent 5,656,271 dated Aug 12 1997
