Clone 15 HL-60细胞
器官来源： 外周血
生长状态： 悬浮生长
ATCC Number： CRL-1964™
相关**： 其他**
年限： 36 years
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 人
细胞形态： **样
数量： 大量
Designations: Clone 15 HL-60
Depositors: S Fischkoff
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Clone 15 HL-60细胞Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Organ: peripheral blood
Disease: acute promyelocytic leukemia
Cellular Products: eosinophil granule proteins: eosinophil major basic protein (MBP), eosinophil peroxidase (EPO), eosinophil cationic protein (ECP) and eosinophil derived neurotoxin (EDN)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1984
Receptors: complement, expressed
Fc, expressed
interleukin 5 (IL-5), expressed
Reverse Transcript: negative
DNA Profile (STR): Amelogenin: X
CSF1PO: 13,14
D13S317: 8,11
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 7,8
TPOX: 8,11
vWA: 16
Age: 36 years
Gender: female
Ethnicity: Caucasian
Comments: Clone 15 HL-60细胞Clone 15 was established in 1984 from a clone of HL-60 (ATCC CCL-240) that had been grown at an elevated pH (pH 7.6 to 7.8) for 2 months.
This clone, picked from soft agar, was selected for its ability to undergo eosinophilic differentiation when treated with butyric acid.
Initially, 80% of the treated cells will differentiate as eosinophils.
However, with continued passage the line will gradually exhibit a modest degree of reversion such that a higher percentage of the cells will exhibit neutrophilic differentiation.
The cells express IL-5 receptors and eosinophil granule proteins, and exhibit chemotaxis to eosinophil specific eosinophilotactins.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Clone 15 HL-60细胞Cultures can be maintained by addition or replacement of medium. Start new cultures at 2 X 10(5) viable cells/ml and subculture at 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22787: Fischkoff SA. Graded increase in probability of eosinophilic differentiation of HL-60 promyelocytic leukemia cells induced by culture under alkaline conditions.Clone 15 HL-60细胞 Leuk. Res. 12: 679-696, 1988. PubMed: 3184985
