G-7细胞
品系: Swiss
数量: 大量
组织来源: skeletal muscle
ATCC Number: CRL-1447™
细胞形态: 成肌细胞
生长状态: 贴壁生长
细胞类型: 其他细胞类型
年限: fetus fetus
是否是肿瘤细胞: 0
物种来源: 小鼠
运输方式: 冻存运输
Designations: G-7
Depositors: J Peacock
G-7细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: myoblast
Source: Strain: Swiss
Tissue: skeletal muscle
Cell Type: myoblast myoblast;
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Tumorigenic: Yes
Cytogenetic Analysis: modal number = 40
Age: fetus fetus
Comments: G-7细胞When confluent, the cells fuse to form multinucleated myotubes.
They are slightly responsive to acetylcholine.
The myoblastic component of the population will rapidly become depleted if the cells are allowed to become confluent, since more extensive fusion will take place.
To avoid this, the cells should be subcultured before they become confluent, and they should be recloned periodically with selection for myoblastic clones.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution for 2 to 5 minutes, remove trypsin, add fresh medium, aspirate and dispense into new Bovine Collagen type l (0.03 mg/ml) coated flasks. Differentiation is enhanced by reducing the sera to 2% each.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Doubling Time: 17 to 19 hrs
References: 1063: Christian CN, et al. G-7细胞Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191