S16细胞
年限： neonatal
ATCC Number： CRL-2941™
生长状态： 贴壁生长
细胞形态： 上皮样
运输方式： 冻存运输
组织来源： sciatic nerve
细胞类型： 其他细胞类型
是否是肿瘤细胞： 0
物种来源： 褐鼠
数量： 大量
S16细胞Designations: S16
Depositors: RH Quarles
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus
Morphology: epithelial-like
Source: Tissue: sciatic nerve
Cell type: Schwann cell
Cellular Products: laminin [16172656]
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Isolation: Isolation date: 1989
Antigen Expression: S16细胞Myelin-associated glycoprotein (MAG) [16172655]
Galactocerebroside (GalC) [16172655]
Age: neonatal
Comments: The S16 cell line is derived from a primary culture of Schwann cells that was immortalized by repetitive passaging. High Myelin-associated glycoprotein (MAG) expression was demonstrated by Radioimmunoassay. The S16 cells divide very rapidly, are rounder than normal Schwann cells and elaborate many processes after reaching high density. The cells express galactocerebroside (GalC), sulfatide and P0 glycoprotein, but little or no myelin basic protein. [16172655] [16172656]
In many respects, the S16 and S42 (ATCC CRL-2942) cells, biochemically, resemble Schwann cells at an early stage in their preparation to myelinate and should be useful for investigating the cell biology of MAG and other myelin-related components. [16172656]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: The culture flasks should be treated with 0.1ml/sq. cm of flask surface area with 15ug/ml poly-L-lysine (Sigma Cat. No. P-9155 or equivalent) for at least 2 hours at 37°C. Remove solution and rinse one time with DPBS and allow flask to air dry uncapped and standing upright in a biological cabinet for about 30 minutes before introducing cells.
Volumes used in this protocol are for 75 sq. cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
S16细胞Remove and discard culture medium.
Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (DPBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant.
Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 4 X 10(3) to 6 X 10(4) viable cells/sq. cm is recommended.
Incubate cultures at 37°C. We recommend that you maintain cultures at a cell concentration between 2 X 10(4) and 4 X 10(4) cells/sq. cm.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 twice weekly is recommended.
Interval: Subculture when cell concentration is between 2 x10(4) and 4x10(4) cells/sq. cm.
Medium renewal: Every 2 to 3 days
Preservation: Freeze medium: complete growth medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: approximately 40 hours
Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2002
Recommended serum: S16细胞ATCC 30-2020
0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101
Phosphate-buffered saline: ATCC 30-2200
Cell culture tested DMSO: ATCC 4-X
Erythrosin B vital stain solution: ATCC 30-2404
Other ATCC Cell Lines: CRL
Other ATCC Cell Lines: CRL
Other ATCC Cell Lines: CRL
References: 16172655: Goda S, et al. Expression of the myelin-associated glycoprotein in cultures of immortalized Schwann cells. J. Neurochem. 56(4):1354-1361, 1991. PubMed: 1705958
16172656: Toda K, et al. Biochemical and cellular properties of three immortalized Schwann cell lines expressing different levels of the myelin-associated glycoprotein. J. Neurochem. 63(5):1646-1657, 1994. PubMed: 7523597
16172657: Sasagasako N, et al. Myelin gene expression in immortalized Schwann cells: relationship to cell density and proliferation. J. Neurochem. 66(4): 1432-1439, 1996. PubMed: 8627295
