TGP52细胞
品系： Tg(E1a-1-SV40E)Bri18
器官来源： 胰腺
细胞形态： 上皮样
数量： 大量
生长状态： 贴壁生长
ATCC Number： CRL-2140™
运输方式： 冻存运输
相关**： 其他**
年限： 26 weeks
是否是肿瘤细胞： 0
物种来源： 转基因小鼠
Designations: TGP52
TGP52细胞Depositors: OS Pettengill, D Longnecker
Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus, transgenic deposited as mouse, transgenic
Morphology: epithelial
Source: Organ: pancreas
Strain: Tg(E1a-1-SV40E)Bri18
Disease: pancreatic islet cell tumor; insulinoma
Cellular Products: somatostatin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Restrictions: TGP52细胞Note: These cells are distributed subject to the following: 1.) This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College. 2.) Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. Tel: (603) 646-3675. 3.) In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications.
Age: 26 weeks
Gender: male
Comments: TGP52 is an epithelial like cell line derived from a pancreatic tumor with islet cell morphology (insulinoma) arising in an ***** male transgenic mouse.
The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen.
In culture, TGP52 cells transiently produced insulin in culture and then produced somatostatin.
Current stocks of TGP52 secrete about 9700 pg/ml of somatostatin in 3 days.
Morphologic and differentiation markers of this line appear to fluctuate from time to time in culture.
Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, 90%; heat-inactivated fetal bovine serum, 10%
Subculturing: TGP52细胞Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22323: Pettengill OS, et al. Cell lines derived from pancreatic tumors of Tg(Ela-1-SV40E)Bri18 transgenic mice express somatostatin and T antigen. Carcinogenesis 15: 61-65, 1994. TGP52细胞PubMed: 7904904
