ACT I细胞
运输方式： 冻存运输
数量： 大量
ATCC Number： HB-80™
生长状态： 悬浮生长
是否是肿瘤细胞： 0
物种来源： 小鼠
细胞形态： **样
**类型： IgG1
Designations: ACT I
Depositors: P Parham
Isotype: IgG1
ACT I细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against actin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. ACT I细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with actin from Dictyostelium discoideum.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with actin from prokaryotic organisms at an epitope distinct from that recognized by ACT IV (ATCC HB-81).
The antibody does not cross-react with eukaryotic actins.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ACT I细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 20%
HAT (13.6 mg/L hypoxanthine (0.1 mM), 0.176 mg/L aminopterin (0.4 �M) and 3.8 mg/L thymidine 0.016 mM)
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2001
Recommended serum: ATCC 30-2020
HAT (Hypoxanthine-Aminopterin-Thymidine): ATCC 69-X
References: 986: Simpson PA, et al. ACT I细胞Monoclonal antibodies prepared against Dictyostelium actin: characterization and interactions with actin. J. Cell Biol. 99: 287-295, 1984. PubMed: 6203918
