SC-1细胞
生长状态： 贴壁生长
数量： 大量
器官来源： 胚胎
ATCC Number： CRL-1404™
是否是肿瘤细胞： 0
物种来源： 小鼠
年限： embryo
细胞形态： 成纤维样
运输方式： 冻存运输
Designations: SC-1
SC-1细胞Depositors: JW Hartley
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: fibroblast
Source: Organ: embryo
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. SC-1细胞Please click here for information regarding the specific requirements for shipment to your location.
Age: embryo
Comments: Initial cultures from a thawed ampule may take 10 to 12 days to become confluent.
Tested and found negative for ectromelia virus (mousepox).
Propagation: SC-1细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, add fresh 0.25% trypsin, 0.03% EDTA for 1 to 3 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks.
References: 22696: Hartley JW, Rowe WP. Clonal cells lines from a feral mouse embryo which lack host-range restrictions for murine leukemia viruses. Virology 65: 128-134, 1975. SC-1细胞PubMed: 167514
