Mar Nol细胞
生长状态： 贴壁生长
年限： 15 years
数量： 大量
器官来源： 皮肤
ATCC Number： CRL-1257™
相关**： 马凡氏综合征
运输方式： 冻存运输
细胞形态： 成纤维样
Mar Nol细胞是否是肿瘤细胞： 0
物种来源： 人
Designations: Mar Nol
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: Mar Nol细胞Marfan syndrome
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 15 years
Gender: female
Comments: The tissue donor suffered from Marfan syndrome and floppy mitral valve syndrome.
There was no prior family history of Marfan syndrome.
Propagation: Mar Nol细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Mar Nol细胞Subculture every 8 to 10 days.
