CCD-1137Sk细胞
细胞类型： 成纤维细胞
是否是肿瘤细胞： 0
物种来源： 人
运输方式： 冻存运输
ATCC Number： CRL-2703™
器官来源： 皮肤
生长状态： 贴壁生长
年限： newborn
细胞形态： 成纤维样
数量： 大量
CCD-1137Sk细胞Designations: CCD-1137Sk
Depositors: F.Haq, ATCC
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens deposited as human
Morphology: fibroblast
CCD-1137Sk细胞
Source: Organ: skin; foreskin
Cell Type: fibroblast
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Age: CCD-1137Sk细胞newborn
Gender: male
Comments: The line was established from skin taken from normal foreskin.
Cells senesce after approximately 60 population doublings.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: CCD-1137Sk细胞Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
