L5178-S (LY-S)细胞
细胞类型： 其他细胞类型
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 悬浮生长
运输方式： 冻存运输
细胞形态： **样
ATCC Number： CRL-1723™
数量： 大量
相关**： **瘤
品系： DBA/2
L5178-S (LY-S)细胞器官来源： 胸腺
Designations: L5178-S (LY-S)
Depositors: JZ Beer
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus
Morphology: lymphoblast
L5178-S (LY-S)细胞
Source: Organ: thymus
Strain: DBA/2
Disease: lymphoma
Cell Type: chemically induced
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Tumorigenic: Yes
Comments: This line was derived from the X-ray resistant L5178-R line (ATCC CRL-1722).
The cells are sensitive to X-rays, ultraviolet radiation, heat and some potential anti neoplastic drugs (e.g., cis-PAD).
The parental cell line was derived from the L5178 thymic lymphoma induced by methylcholanthrene in a DBA/2 mouse.
Propagation: ATCC complete growth medium: Fischer's medium with 0.1 g/L sodium pyruvate and 1.125 sodium bicarbonate, 90%; horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp4 cells/ml and maintain between 5 X 10 exp3 and 7 X 10 exp5 cells/ml.
Preservation: culture medium, 90%; DMSO, 10%
References: L5178-S (LY-S)细胞1164: Beer JZ, et al. X-ray and UV mutagenesis in two L5178Y cell strains differing in tumorigenicity, radiosensitivity, and DNA repair. Br. J. Cancer Suppl. 6: 107-111, 1984. PubMed: 6582899
1166: Beer JZ, et al. Loss of tumorigenicity with simultaneous changes in radiosensitivity and photosensitivity during in vitro growth of L5178Y murine lymphoma cells. Cancer Res. 43: 4736-4742, 1983. PubMed: 6883332
58059: Beer JZ, et al. Effects of low dose rate (0.003-0.025 Gy/h) chronic X-irradiation on radioresistant and radiosensitive L5178Y mouse lymphoma cells. Int. J. Radiat. Biol. 48: 609-619, 1985. PubMed: 3876312
