RSOI细胞
细胞形态： 成纤维样
数量： 大量
年限： 7 years
是否是肿瘤细胞： 0
物种来源： 人
ATCC Number： CRL-1419™
相关**： 成骨不全症
生长状态： 贴壁生长
运输方式： 冻存运输
器官来源： 皮肤
Designations: RSOI
RSOI细胞Depositors: GR Martin
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
RSOI细胞
Source: Organ: skin
Disease: osteogenesis imperfecta
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 7 years
Gender: female
Comments: RSOI细胞The tissue donor suffered from osteogenesis imperfecta.
There was no prior family history of osteogenesis imperfecta.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. RSOI细胞Subculture every 7 to 9 days.
