4D12细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 悬浮生长
运输方式： 冻存运输
数量： 大量
细胞形态： **样
**类型： IgG1
ATCC Number： HB-178™
Designations: 4D12
Depositors: BF Haynes
Isotype: IgG1
4D12细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
4D12细胞B
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against HLA B5
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Comments: 4D12细胞Animals were immunized with the HUT 78 Sezary cutaneous T cell line.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody reacts with an HLA determinant that includes specificities within, but not identical to, the B5 cross-reactive group.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 80%; heat-inactivated fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1193: Haynes BF, et al. Description of monoclonal antibody defining an HLA allotypic determinant that includes specificities within the B5 cross-reacting group. Hum. Immunol. 4: 273-285, 1982. 4D12细胞PubMed: 6181035
