VM-2细胞
生长状态： 悬浮生长
运输方式： 冻存运输
数量： 大量
ATCC Number： HB-8530™
细胞形态： **样
**类型： IgG1
是否是肿瘤细胞： 0
物种来源： 小鼠
Designations: VM-2
VM-2细胞Depositors: Stanford University
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human basal cells (skin)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. VM-2细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with human epidermal cells.
Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.
The antibody binds to a cell surface antigen present on and characteristic of human basal cells and malignant squamous cells.
The antibody does not react with normal human fibroblasts, endothelial or hematopoietic cells.
VM-2细胞Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine, 0.45 mM sodium pyruvate and 0.01 M HEPES, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium, 90%; DMSO, 10%
References: 3933: Morhenn V. VM-2细胞Monoclonal antibody specific for human basal cell surface antigen. US Patent 4,886,745 dated Dec 12 1989
