EGC/PK060399egfr细胞
器官来源： 其他
细胞类型： 其他细胞类型
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 褐鼠
品系： Sprague-Dawley
组织来源： myenteric plexus
数量： 大量
EGC/PK060399egfr细胞细胞形态： 其他
年限： ***** *****
ATCC Number： CRL-2690™
生长状态： 贴壁生长
Designations: EGC/PK060399egfr
Depositors: A Ruhl
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: EGC/PK060399egfr细胞Rattus rattus deposited as rat
Morphology: glial
Source: Organ: jejunum
Strain: Sprague-Dawley
Tissue: myenteric plexus
Cell Type: enteroglial;
Cellular Products: glial fibrillary acidic protein (GFAP); S-100 protein and vimentin [53238]
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: March 6, 1999
EGC/PK060399egfr细胞Age: ***** *****
Gender: male
Comments: The cells display robust glial fibrillary acidic protein (GFAP), S-100 and vimentin immunoreactivities, but no Thy-1.1, desmin, smooth muscle alpha-actin or C3 complement receptor immunoreactivity. [53238]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: EGC/PK060399egfr细胞Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 53238: Ruhl A, et al. Isolation of enteric glia and establishment of transformed enteroglial cell lines from the myenteric plexus of ***** rat. Neurogastroenterol. Motil. 13: 95-106, 2001. EGC/PK060399egfr细胞PubMed: 11169131
