BLK CL.4细胞
细胞形态： 成纤维样
生长状态： 贴壁生长
ATCC Number： TIB-81™
数量： 大量
相关**： 正常
品系： C57BL/6
细胞类型： 成纤维细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
运输方式： 冻存运输
BLK CL.4细胞年限： embryo
Designations: BLK CL.4
Depositors: P Patek, J Collins, M Cohn
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: fibroblast
Source: Disease: normal
Strain: C57BL/6
Cell Type: fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material.BLK CL.4细胞 Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: No
Age: embryo
Comments: These cells do not grow in 0.3% agarose.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. BLK CL.4细胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove old medium, add fresh 0.25% trypsin for 1 minute, remove trypsin and let the culture sit at room temperature until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
References: 22905: Patek PQ, et al. Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. BLK CL.4细胞PubMed: 723934
