DH82细胞
是否是肿瘤细胞： 0
物种来源： 狗
数量： 大量
生长状态： 贴壁生长
运输方式： 冻存运输
细胞形态： 单核细胞/巨噬细胞
年限： 10 years
ATCC Number： CRL-10389™
相关**： 其他**
Designations: DH82
Depositors: The United States of America
DH82细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Canis familiaris
Morphology: macrophage
Source: Disease: malignant histiocytosis
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Age: 10 years
Gender: male
Comments: DH82细胞The DH82 cell line has a macrophage-like morphology, and is able to phagocytize latex particles.
They are positive for Fc gamma receptors and negative for Fc mu and C3b receptors.
The cells do not produce IL-1.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%.
Temperature: 37.0°C
Subculturing: DH82细胞Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: DH82细胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
References: 22019: Dawson JE, Rikihisa Y. Growing Ehrlichia species in a continuous cell line. US Patent 5,192,679 dated Mar 9 1993
22675: Wellman ML, et al. A macrophage-monocyte cell line from a dog with malignant histiocytosis. In Vitro Cell. Dev. Biol. 24: 223-229, 1988. PubMed: 3350786
