HRT-18G细胞
细胞形态： 上皮样
运输方式： 冻存运输
是否是肿瘤细胞： 0
物种来源： 人
数量： 大量
生长状态： 贴壁生长
器官来源： 结肠
年限： 67 years
ATCC Number： CRL-11663™
HRT-18G细胞相关**： 其他**
Designations: HRT-18G
Depositors: Louisiana State Univ. Agricultural and Mechanical College
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: colon
Disease: HRT-18G细胞ileocecal colorectal adenocarcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: They can be used for propagation and plaque titration of bovine coronaviruses.
Age: 67 years
Gender: male
Comments: This line is a derivative of HRT-18 (ATCC CCL-244) and represents a subpopulation of cells selected by successive passage in different media.
HRT-18G cells show enhanced susceptibility to bovine coronaviruses relative to the parental HRT-18 cell line.
They can be used for propagation and plaque titration of bovine coronaviruses.
Propagation: HRT-18G细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended
Medium Renewal: Twice per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Preservation: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: HRT-18G细胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
Recommended serum: ATCC 30-2020
0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101
Cell culture tested DMSO: ATCC 4-X
Parental cell line: ATCC CCL-244
