HBE4-E6/E7-C1细胞
器官来源： 肺
数量： 大量
细胞形态： 上皮样
年限： 60 years
细胞类型： 其他细胞类型
是否是肿瘤细胞： 0
物种来源： 人
运输方式： 冻存运输
组织来源： bronchus
生长状态： 贴壁生长
HBE4-E6/E7-C1细胞ATCC Number： CRL-2079™
Designations: HBE4-E6/E7-C1 [NBE4-E6/E7-C1]
Depositors: J Viallet
Biosafety Level: 2 [Cells contain human papilloma viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: lung
Tissue: bronchus
Cell Type: HBE4-E6/E7-C1细胞epithelial; human papillomavirus 16 (HPV-16) E6/E7 transformed
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cytogenetic Analysis: 45, X, -Y, dup (5), -8, +9, -14, -15, -20, -21, -22, +mar1, +mar2, +der(8q;13q)
Age: 60 years
Gender: male
Ethnicity: Caucasian
Comments: The HBE4-E6/E7-C1 (formerly NBE4-E6/E7-C1) cell line was cloned from passage 22 of HBE4-E6/E7 (see ATCC CRL-2078) by limiting dilution.
The cloned line is more sensitive than the parental line to induction of terminal differentiation by phorbol esters.
The cells are non-viable in DMSO and should be frozen in culture medium with 10% glycerol.
Propagation: HBE4-E6/E7-C1细胞ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
0.05 mg/ml BPE - provided with the K-SFM kit
5 ng/ml EGF - provided with the K-SFM kit
10 ng/ml cholera toxin - not provided with kit
NOTE: Do not filter complete medium
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution containing 0.5% polyvinylpyrrolidone (PVP).
Add 2.0 to 3.0 ml of Trypsin-EDTA-PVP solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: HBE4-E6/E7-C1细胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 2.0 to 3.0 ml of complete growth medium containing 0.1% soybean trypsin inhibitor and 0.1% bovine serum albumin and aspirate cells by gently pipetting.Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant.
Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.
Subculture before the cells become confluent.
Preservation: culture medium, 90%; glycerol, 10%
Doubling Time: 24 hrs
References: 22447: Viallet J, et al. Characterization of human bronchial epithelial cells immortalized by the E6 and E7 genes of human papillomavirus type 16. Exp. Cell Res. 212: 36-41, 1994. HBE4-E6/E7-C1细胞PubMed: 8174640
