HeLa细胞
器官来源： 宫颈
生长状态： 贴壁生长
年限： 31 years *****
运输方式： 冻存运输
ATCC Number： CCL-2™
相关**： 腺癌
数量： 大量
细胞形态： 上皮样
细胞类型： 上皮细胞
是否是肿瘤细胞： 0
物种来源： 人
HeLa细胞Designations: HeLa
Depositors: WF Scherer
Biosafety Level: 2 [Cells contain human papilloma virus ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: cervix
Disease: adenocarcinoma
Cell Type: epithelial
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:HeLa细胞 transfection host
screening for Escherichia coli strains with invasive potential
Virus Susceptibility: Human adenovirus 3
Encephalomyocarditis virus
Human poliovirus 1
Human poliovirus 2
Human poliovirus 3
DNA Profile (STR): Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Cytogenetic Analysis: Modal number = 82; range = 70 to 164.
There is a small telocentric chromosome in 98% of the cells. 100% aneuploidy in 1385 cells examined. Four typical HeLa marker chromosomes have been reported in the literature. HeLa Marker Chromosomes: One copy of Ml, one copy of M2, four-five copies of M3, and two copies of M4 as revealed by G-banding patterns. HeLa细胞M1 is a rearranged long arm and centromere of chromosome 1 and the long arm of chromosome 3. M2 is a combination of short arm of chromosome 3 and long arm of chromosome 5. M3 is an isochromosome of the short arm of chromosome 5. M4 consists of the long arm of chromosome 11 and an arm of chromosome 19.
Isoenzymes: G6PD, A
Age: 31 years *****
Gender: female
Ethnicity: Black
HeLa Markers: Y
Comments: The cells are positive for keratin by immunoperoxidase staining.
HeLa cells have been reported to contain human papilloma virus 18 (HPV-18) sequences.
P53 expression was reported to be low, and normal levels of pRB (retinoblastoma suppressor) were found.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
HeLa细胞Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37�C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
also available as Certified Reference Material, ATCC CRM-CCL-2
derivative:ATCC CCL-2.1
derivative:ATCC CCL-2.2
derivative:ATCC CCL-2.3
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