RSC96细胞
ATCC Number： CRL-2765™
细胞形态： 神经元
生长状态： 贴壁生长
数量： 大量
细胞类型： 其他细胞类型
是否是肿瘤细胞： 0
物种来源： 褐鼠
运输方式： 冻存运输
Designations: RSC96
Depositors: PI Patel
RSC96细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus
Morphology: neuronal
Source: Cell Type: neuronal Schwann cell; spontanous immortalization
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Comments: Oncogene:
Eerb-B2, positive RSC96细胞[PubMed: 9766530]Erb-B3, negative [PubMed: 9766530]Products:
cyclic nucleotide phosphodiesterase 1 (Cnp1) (CNPase); positive[PubMed: 9766530]laminin; positive [PubMed: 9766530]Myelin-associated glycoprotein (Mag); Protein and mRNA, negative [PubMed: 12210843] Myelin basic protein (Mbp); Protein and mRNA, negative [PubMed: 12210843]Myelin protein zero (Mpz) (Charcot-Marie-Tooth neuropathy 1B); Protein and mRNA, negative [PubMed: 12210843]peripheral myelin protein 22 (Pmp22); mRNA, positive; protein, negative [PubMed: 12210843]S100 calcium-binding protein, beta (neural) (S100b) protein, positive [PubMed: 12210843]Receptors expressed:
Nerve growth factor receptor (Ngfr); Protein and mRNA, negative [PubMed: 12210843]platelet derived growth factor receptor, alpha; negative [PubMed: 9766530]platelet derived growth factor receptor, beta; negative [PubMed: 9766530]
Comments:
The RSC96 cell line is a spontaneously transformed rat Schwann cell line derived from long-term culture of rat primary Schwann cells [PubMed: 9766530].A culture submitted to the ATCC in December of 2002 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.The cells were assayed for mycoplasma, by the Hoechst stain, PCR and the standard culture test, after a six-week period following treatment. All tests were negative.
Propagation: RSC96细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37�C.
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended
Medium Renewal: Two to three times weekly
Preservation: Freeze medium:RSC96细胞 Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 64424: Hai M, et al. Comparative analysis of Schwann cell lines as model systems for myelin gene transcription studies. J. Neurosci. Res. 69: 497-508, 2002. PubMed: 12210843
71856: Badache A, De Vries GH. Neurofibrosarcoma-derived Schwann cells overexpress platelet-derived growth factor (PDGF) receptors and are induced to proliferate by PDGF BB. J. Cell. Physiol. 177: 334-342, 1998. PubMed: 9766530
