PU5-1.8 (PU5-1R)细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
生长状态： 悬浮生长，少量贴壁
运输方式： 冻存运输
品系： BALB/c
ATCC Number： TIB-61™
数量： 大量
相关**： 其他**
Designations: PU5-1.8 (PU5-1R)
Depositors: P Ralph
PU5-1.8 (PU5-1R)细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension (some adherent cells)
Organism: Mus musculus
Morphology:
Source: Disease: lymphoid tumor
Strain: BALB/c
Cellular Products: lysozyme; granulocyte colony stimulating activity (CSA) inducible by LPS
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. PU5-1.8 (PU5-1R)细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors: complement (C3)
Comments: PU5-1.8 cells phagocytose latex beads and zymosan.
The cells are capable of antibody dependent lysis of both sheep erythrocytes and tumor cells.
The line is sensitive to growth inhibition by LPS and PPD.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing: PU5-1.8 (PU5-1R)细胞Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Adherent cells can be recovered by scraping.
References: 1080: Ralph P, et al. Lysozyme synthesis by established human and murine histiocytic lymphoma cell lines. J. Exp. Med. 143: 1528-1533, 1976. PubMed: 1083890
1135: Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031
1136: Ralph P, Nakoinz I. Direct toxic effects of immunopotentiators on monocytic myelomonocytic, and histiocytic or macrophage tumor cells in culture. Cancer Res. 37: 546-550, 1977. PU5-1.8 (PU5-1R)细胞PubMed: 318922
1137: Ralph P, Nakoinz I. Lipopolysaccharides inhibit lymphosarcoma cells of bone marrow origin. Nature 249: 49-51, 1974. PubMed: 4208429
