SFR8-B6细胞
数量: 大量
细胞形态: **样
**类型: IgG2b
运输方式: 冻存运输
生长状态: 悬浮生长
ATCC Number: HB-152™
是否是肿瘤细胞: 0
物种来源: 大鼠
Designations: SFR8-B6
Depositors: SF Radka
Isotype: IgG2b
SFR8-B6细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against HLA Bw6
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Comments: Animals were immunized with partially purified glycoprotein preparations from detergent solubilized membranes from the JY human lymphoblastoid cell line.
Popliteal lymph node cells were fused with Sp2/0-Ag14 myeloma cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
SFR8-B6细胞fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Temperature: 37.0°C
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 1002: Radka SF, et al. A monoclonal antibody directed against the HLA-BW6 epitope. J. Immunol. 128: 2804-2806, 1982. PubMed: 6176653
32497: White CA, et al. Constitutive transduction of peptide transporter and HLA genes restores antigen processing function and cytotoxic T cell-mediated immune recognition of human melanoma cells. Int. J. Cancer 75: 590-595, 1998. SFR8-B6细胞PubMed: 9466661