PG13/LN c8细胞
是否是肿瘤细胞： 0
物种来源： 小鼠
运输方式： 冻存运输
生长状态： 贴壁生长
品系： NIH/Swiss
细胞形态： 成纤维样
年限： embryo
ATCC Number： CRL-10685™
数量： 大量
相关**： 正常
Designations: PG13/LN c8
PG13/LN c8细胞Depositors: Fred Hutchinson Cancer Res. Cntr.
Biosafety Level: 2 [CELLS CONTAIN RETROVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: fibroblast
Source: Disease: normal
Strain: NIH/Swiss
Cellular Products: a Gibbon ape Leukemia Virus (GaLV) based neomycin resistance transducing vector
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. PG13/LN c8细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Reverse Transcript: positive
Age: embryo
Comments: PG13/LN c8 cells produce a retroviral vector (LN) that transduces the neo gene and can infect cells from many mammalian species (other than mice).
The line was derived from PG13 (see ATCC CRL-10686).
Although virus production has not been observed, there is a possibility that the cells will produce a virus similar to GaLV (a moderate risk oncogenic virus) and Biosafety Level 2 or higher precautions should be taken when using this cell line.
Propagation: ATCC complete growth medium: PG13/LN c8细胞The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% Trypsin, 0.02% EDTA solution and allow the flask to sit at room temperature (or 37C) until the cells detach (2 to 3 minutes). PG13/LN c8细胞Add fresh medium, aspirate and dispense into new flasks.
References: 1818: Miller AD, et al. Construction and properties of retrovirus packaging cells based on gibbon ape leukemia virus. J. Virol. 65: 2220-2224, 1991. PubMed: 1850008
4473: Miller AD, Rosman GJ. Improved retroviral vectors for gene transfer and expression. BioTechniques 7: 980-990, 1989. PubMed: 2631796
