Da Bon细胞
是否是肿瘤细胞： 0
物种来源： 人
数量： 大量
生长状态： 贴壁生长
ATCC Number： CRL-1251™
相关**： 成骨不全症
器官来源： 皮肤
年限： 2 years
细胞形态： 成纤维样
运输方式： 冻存运输
Designations: Da Bon
Da Bon细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: osteogenesis imperfecta (tarda)
Permits/Forms: Da Bon细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 2 years
Gender: female
Comments: The tissue donor suffered from osteogenesis imperfecta (tarda), and was the daughter of Ro Bon (ATCC CRL-1248).
Propagation: Da Bon细胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at room temperature for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Da Bon细胞Subculture every 6 to 8 days.
