DBS-FRhL-2细胞
生长状态： 贴壁生长
数量： 大量
运输方式： 冻存运输
细胞类型： 成纤维细胞
细胞形态： 成纤维样
是否是肿瘤细胞： 0
物种来源： 猕猴
ATCC Number： CL-160™
相关**： 正常
年限： fetus
DBS-FRhL-2细胞器官来源： 肺
Designations: DBS-FRhL-2
Depositors: U.S. Government
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Macaca mulatta
Morphology: fibroblast
Source: Organ: lung
Disease: normal
Cell Type: fibroblast
Permits/Forms: DBS-FRhL-2细胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: April, 1969
Tumorigenic: No
Cytogenetic Analysis: Model number = 42. Range = 37 to 46.Seventy percent of the cells are diploid and the karyotype is that of a male rhesus monkey. No structural abnormalities are evident.
Age: fetus
Gender: male
Comments: This line was formerly designated CCL-160.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: DBS-FRhL-2细胞Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 3522: Hopps HE, et al. Sub-human primate diploid cell lines as substrates for virus vaccine production. US Patent 4,040,905 dated Aug 9 1977
22396: Wallace RE, et al. DBS-FRhL-2细胞Development of a diploid cell line from fetal rhesus monkey lung for virus vaccine production. In Vitro 8: 323-332, 1973. PubMed: 4348695
