4E8C12细胞
Designations: 4E8C12
Depositors: Wisconsin Alumni Res. Fndn.
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
4E8C12细胞Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against an enterohemorrhagic strain of Escherichia coli (E. coli 0157:H7 strain 932)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with a rough strain of 0157:H7 4E8C12细胞(E. coli HA1, deficient in the 0157 antigen).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for E coli 0157:H7 and 026:H11 both of which are enterohemorrhagic.
It reacts with a protein antigen of 13000 daltons.
Propagation: 4E8C12细胞ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 50 units/ml penicillin, 0.05 mg/ml streptomycin and 0.05 mg/ml gentamicin, 80%; fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: culture medium, 90%; DMSO, 10%
References: 22008: Doyle MP, Padhye N. Monoclonal antibody to enterohemorrhagic Escherichia coli 0157:H7 and 026:H11. US Patent 5,168,063 dated Dec 1 1992
22063: Doyle MP, Padhye N. 4E8C12细胞Monoclonal antibody to enterohemorrhagic Escherichia coli 0157:H7 and 026:H11 and method for detection. US Patent 5,354,661 dated Oct 11 1994
