CCD-11Lu细胞
细胞类型： 成纤维细胞
是否是肿瘤细胞： 0
物种来源： 人
器官来源： 肺
数量： 大量
生长状态： 贴壁生长
运输方式： 冻存运输
年限： 9 years
ATCC Number： CCL-202™
相关**： 正常
CCD-11Lu细胞细胞形态： 成纤维样
Designations: CCD-11Lu
Depositors: GP Maxwell
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: lung
Disease: normal
Cell Type: CCD-11Lu细胞fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Antigen Expression: blood type O; Rh +
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 11
D13S317: 8,14
D16S539: 9,12
D5S818: 11,13
D7S820: 10,12
THO1: 9
TPOX: 8,11
vWA: 18,20
Cytogenetic Analysis: normal human male; diploid; stable
Age: 9 years
Gender: male
Ethnicity: Asian
Comments: CCD-11Lu细胞The line was derived from normal tissue from patient who died following trauma to the head.
The cells are capable of at least 12 population doublings beyond the current freeze.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin for several minutes, remove trypsin and let the culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks. CCD-11Lu细胞Subculture every 5 to 7 days.
