MM.1S细胞
细胞类型： 其他细胞类型
组织来源： neoplastic
是否是肿瘤细胞： 0
物种来源： 人
ATCC Number： CRL-2974™
相关**： immunoglobulin A lambda myeloma [
生长状态： 混合型生长
数量： 大量
细胞形态： **样
年限： 42 years [16173231]
MM.1S细胞器官来源： 外周血
运输方式： 冻存运输
Designations: MM.1S
Depositors: S. Rosen
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: mixed, suspension and lightly attached cells
Organism: Homo sapiens
Morphology: lymphoblast
Source: Organ: peripheral blood
Tissue State: neoplastic
Cell Type: B lymphoblast
Disease:MM.1S细胞 immunoglobulin A lambda myeloma [16173230]
Cellular Products: lambda-light chain immunoglobulin [16173231]
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1986
cloning method: soft agar
Receptors: glucocorticoid receptor, expressed
Antigen Expression: CD25+, CD38+, CD52+, CD59+ [16173231]
DNA Profile (STR): D5S818: 8, 12
D13S317: 13
D7S820: 10, 11
D16S539: 12
vWA: 15, 17
THO1: 7, 8
TPOX: 8
CSF1PO: 9,14
Amelogenin: X
Age: 42 years MM.1S细胞[16173231]
Gender: female
Ethnicity: Black
Comments: MM.1S is sensitive to dexamethasone. The parent cell line, MM.1, was established from peripheral blood of a multiple myeloma patient who had become resistant to steroid-based therapy. The closely related cell line, MM.1R (ATCC CRL-2975) was also isolated from MM.1, but is resistant to dexamethasone. Together, CRL-2974 and CRL-2975 provide critical information about disease progression and the development of drug resistance. These cell lines represent valuable tools for elucidating the mechanisms of action of glucocorticoids and the development of new therapeutics.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol:
Subculture cells before they reach confluence; cells in over-confluent cultures begin to form rosettes with necrotic centers.
This is a mixed cell culture; cells grow both as a lightly attached monolayer and in suspension. Cultures can be maintained by adding fresh medium. Alternatively, subcultures can be prepared by scraping the adherent cells into the medium containing the floating cells, collecting the cells by centrifugation, resuspending the cell pellet in fresh medium and dispensing into new flasks.
Subcultivation ratio: A subcultivation ratio of 1:2 to 1:4 is recommended.
Medium renewal: Every 2 to 3 days
Preservation: MM.1S细胞Freeze medium: complete growth medium, 90%; DMSO, 10%
liquid nitrogen vapor phase
Doubling Time: approximately 72 hours
Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2001
Recommended serum: ATCC 30-2020
Cell culture tested DMSO: ATCC 4-X
Derived from same cell line: ATCC CRL-2975
References: 16173230: Goldman-Leikin RE, et al. Characterization of a novel myeloma cell line, MM.1. J. Lab. Clin. Med. 113(3):335-345, 1989. PubMed: 2926241
16173231: Greenstein S, et al. Characterization of the MM.1 human multiple myeloma (MM) cell lines: A model system to elucidate the characteristics, behavior, and signaling of steroid-sensitive and -resistant MM cells. Exp. Hematol.: 31:271-282, 2003. PubMed; 12691914
16173308: Moalli PA, et al. A mechanism of resistance to glucocorticoids in multiple myeloma: transient expression of a truncated glucocorticoid receptor mRNA. Blood. 79:213-222, 1992. PubMed: 1728309
