SK-PN-DW细胞
ATCC Number： CRL-2139™
相关**： 其他**
细胞形态： 上皮样
是否是肿瘤细胞： 0
物种来源： 人
器官来源： 其他
数量： 大量
生长状态： 贴壁生长
年限： 17 years
运输方式： 冻存运输
Designations: SK-PN-DW
Depositors: C Helson
SK-PN-DW细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: retroperitoneal embryonal tumor
Disease: malignant primitive neuroectodermal tumor
Cellular Products: cholecystokinin (CCK)
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Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 12
D13S317: 9
D16S539: 12
D5S818: 13,14
D7S820: 8,14
THO1: 6
TPOX: 8,9
vWA: 16
Cytogenetic Analysis: aneuploid; modal number = 40 to 41; monosomy 13 was found in 100% of the cells; iso(17q) and monosomy 9, 10, 11, and 18 were found in greater than 20% of cells analyzed., In a large proportion of the cells, the double minutes per cell were too numerous to count.
Age: 17 years
Gender:SK-PN-DW细胞 male
Ethnicity: Caucasian
Comments: SK-PN-DW is a primitive neuroectodermal tumor (PNET) cell line derived in 1979 from a 17 year old Caucasian male with embryonal retroperitoneal tumor.
The cells express CCK specific mRNA and synthesize considerable quantities of variably processed CCK prohormone.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 is recommended
Medium Renewal: SK-PN-DW细胞Twice per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 22258: Potluri VR, et al. Primitive neuroectodermal tumor cell lines: chromosomal analysis of five cases. Cancer Genet. Cytogenet. 24: 75-86, 1987. PubMed: 3024811
22763: Schneider BS, et al. Expression of the cholecystokinin gene by cultured human primitive neuroepithelioma cell lines. J. Clin. Endocrinol. Metab. 69: 411-419, 1989. PubMed: 2753982
