B14FAF28-G3细胞
生长状态： 贴壁生长
数量： 大量
年限： *****
细胞形态： 上皮样
器官来源： 其他
ATCC Number： CCL-14™
运输方式： 冻存运输
相关**： 正常
是否是肿瘤细胞： 0
物种来源： 仓鼠
B14FAF28-G3细胞Designations: B14FAF28-G3
Depositors: TC Hsu
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Cricetulus griseus
Morphology: epithelial
Source: Organ: peritoneum
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. B14FAF28-G3细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance: poliovirus 1, 3; adenovirus 3
Age: *****
Gender: female
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
B14FAF28-G3细胞Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Twice per week
Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and let culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
References: 22296: . . Science 133: 1600, 1961.
26032: Hsu TC, et al. B14FAF28-G3细胞Radiosensitivity of cells of Chinese hamster in vitro in relation to the cell cycle. Exp. Cell Res. 27: 441-452, 1962. PubMed: 13955320
