WR19M.1细胞
是否是肿瘤细胞: 0
物种来源: 小鼠
数量: 大量
生长状态: 贴壁生长
运输方式: 冻存运输
ATCC Number: TIB-70™
相关**: 其他**
品系: BALB/c
Designations: WR19M.1
WR19M.1细胞Depositors: WC Raschke
Biosafety Level: 2
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology:
Source: Disease: Abelson murine leukemia virus-induced tumor
Strain: BALB/c
Cellular Products: lysozyme; Abelson murine leukemia virus (infectious)
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Receptors: complement (C3)
Antigen Expression: H-2d
Gender: female
Comments: This line was derived from the same tumor as WR19L (ATCC TIB-52).
The cells will pinocytose neutral red and phagocytose zymosan and latex beads.
They will also lyse antibody coated sheep erythrocytes.
They are negative for surface immunoglobulin (sIg-), Ia (Ia-) and Thy-1.2 (Thy-1.2-).
WR19M.1细胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; horse serum, 10%
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: 1 to 2 times per week
Subcultures are prepared by scraping. Remove old medium, add fresh, dislodge cells and dispense into new flasks.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum: ATCC 30-2040
References: 1207: Raschke WC, et al. WR19M.1细胞Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198