K6H6/B5细胞
器官来源： **结
是否是肿瘤细胞： 0
物种来源： 人
生长状态： 悬浮生长
运输方式： 冻存运输
数量： 大量
ATCC Number： CRL-1823™
相关**： 其他**
细胞形态： **样
Designations: K6H6/B5
K6H6/B5细胞Depositors: R Levy
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: human (B cell lymphoma); mouse (myeloma)
Morphology: lymphoblast
Source: Organ: lymph node
Disease: nodular lymphoma
Cell Type: hybridoma: B lymphocyte; somatic cell hybrid
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. K6H6/B5细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: The line was produced from a fusion of P3/NSI/1-Ag4-1 cells with malignant cells from a human nodular lymphoma.
The cells are deficient in hypoxanthine phosphoribosyltransferase (HPRT) and are excellent fusion partners for human B cells.
Originally the cells secreted human IgM with a lambda light chain; however, with continuous passage they spontaneously lost the ability to secrete immunoglobulin.
Tested and found negative for ectromelia virus (mousepox).
Comments: The line was produced from a fusion of P3/NSI/1-Ag4-1 cells with malignant cells from a human nodular lymphoma. Originally the cells secreted human IgM with a lambda light chain; however, with continuous passage they spontaneously lost the ability to secrete immunoglobulin. The cells are deficient in hypoxanthine phosphoribosyltransferase (HPRT) and are excellent fusion partners for human B cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: K6H6/B5细胞Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2001
Recommended serum: ATCC 30-2020
Cell culture tested DMSO: ATCC 4-X
Erythrosin B vital stain solution: ATCC 30-2404
References: 1625: Carroll WL, et al. K6H6/B5细胞Mouse X human heterohybridomas as fusion partners with human B cell tumors. J. Immunol. Methods 89: 61-72, 1986. PubMed: 3084658
