Sf9细胞
生长状态： 混合型生长
运输方式： 冻存运输
ATCC Number： CRL-1711™
器官来源： 卵巢
细胞形态： 上皮样
是否是肿瘤细胞： 0
物种来源： 其他
年限： pupa
数量： 大量
Designations: Sf9
Sf9细胞Depositors: G Smith, C Cherry, MD Summers
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: mixed: adherent/suspension
Organism: Spodoptera frugiperda
Morphology: epithelial
Source: Organ: ovary
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Isolation: Sf9细胞Isolation date: 1983
Applications: transfection host
Virus Susceptibility: Nuclear polyhedrosis virus, Autographa californica
St. Louis encephalitis virus
Age: pupa
Gender: female
Comments: This line can be used to replicate baculovirus expression vectors.
It is important to use the medium described below.
Propagation: ATCC complete growth medium: The base medium for this cell line is Grace's Insect Medium Supplemented (GIBCO/Invitrogen Cat. No. 11605-094 or equivalent). To make the complete growth medium, add the following components to the base medium: heat-inactivated insect cell culture tested fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%
Temperature: 28.0°C
Growth Conditions: Sf9细胞The recommended media are formulated for use without CO2. Omission of the yeastolate or lactalbumin hydrolysate will lead to poor performance by this line.
Subculturing: Protocol: Gently resuspend cells in the spent culture medium by pipetting across the monolayer or by hitting the flask against the palm of your hand (the latter is only preferable when working with larger flasks).
Subcultivation Ratio: A subcultivation ratio of 1:5 or greater is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
References: 22650: Vaughn JL, et al. The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera; Noctuidae). In Vitro 13: 213-217, 1977. PubMed: 68913
23136: Zhang PF, et al. Susceptibility of the Sf9 insect cell line to infection with adventitious viruses. Biologicals 22: 205-213, 1994. PubMed: 7811453
23294: Smith GE, et al. Modification and secretion of human interleukin 2 produced in insect cells by a baculovirus expression vector. Proc. Natl. Acad. Sci. USA 82: 8404-8408, 1985. PubMed: 3878519
32359: Hall MR, Gibson W. Cytomegalovirus assemblin: the amino and carboxyl domains of the proteinase form active enzym e when separately cloned and coexpressed in eukaryotic cells. J. Virol. 70: 5395-5404, 1996. PubMed: 8764050
32361: Bakker A, et al. Human T-cell leukemia virus type 2 Rex inhibits pre-mRNA splicing in vitro at an early stage of spliceosome formation. J. Virol. 70: 5511-5518, 1996. PubMed: 8764063
32693: Gibson W, et al. Cytomegalovirus "missing" capsid protein identified as heat-aggregable product of human cytomegalovirus UL46. J. Virol. 70: 7454-7461, 1996. PubMed: 8892863
32860: Filtz TM, et al. Purification and G protein subunit regulation of a phospholipase C-beta from Xenopus laevis oocytes. J. Biol. Chem. 49: 31121-31126, 1996. PubMed: 8940109
32882: Lindorfer MA, et al. G protein gamma subunits with altered prenylation sequences are properly modified when expressed in Sf9 cells. J. Biol. Chem. 271: 18582-18587, 1996. Sf9细胞PubMed: 8702508
33023: Bruant SS, et al. N-terminal sequences contained in the Src homology 2 qand 3 domains of p120 GTPase-activating protein are required for full catalytic activity toward ras. J. Biol. Chem. 271: 5195-5199, 1996. PubMed: 8617802
33034: Stewart L, et al. Biochemical and biophysical analyses of recombinant forms of human topoisomerase I. J. Biol. Chem. 271: 7593-7601, 1996. PubMed: 8631793
33143: Kawabe J, et al. Soluble adenylyl cyclase from Spodoptera frugiperda (Sf9) cells. J. Biol. Chem. 271: 20132-20137, 1996. PubMed: 8702736
33160: Yasuda H, et al. Role of the prenyl group on the G protein gamma subunit in coupling trimeric G proteins to A1 adenosine receptors. J. Biol. Chem. 271: 18588-18595, 1996. PubMed: 8702509
