AR42J细胞
运输方式： 冻存运输
细胞形态： 上皮样
数量： 大量
品系： Wistar
组织来源： exocrine
生长状态： 贴壁生长
器官来源： 胰腺
是否是肿瘤细胞： 0
物种来源： 大鼠
ATCC Number： CRL-1492™
相关**： 肿瘤
Designations: AR42J
Depositors: NW Jessop
AR42J细胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology: epithelial
Source: Organ: pancreas
Strain: Wistar
Tissue: exocrine
Disease: tumor
Cellular Products: amylase and other exocrine enzymes [22185]
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. AR42J细胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host
Receptors: insulin, expressed
glucocorticoid, expressed
Tumorigenic: Yes
Comments: Secretory activity is inducible by glucocorticoid stimulation, and is accompanied by extensive re-organization of the endoplasmic reticulum.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20% .
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Growth Conditions: The cells cells grow slowly, in clusters. They tend to pile up and appear refractile.
Subculturing: Protocol: Monolayer never becomes confluent. Subculture when patches of cells start forming "domes".
Remove and discard culture medium.
AR42J细胞Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37�C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximately 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.
Incubate cultures at 37�C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 3 to 4 days. May need to only add media initially, do not fluid change until cells attach well.
Preservation: Freeze medium: Complete growth medium supplemented with an additional 30% (v/v) fetal bovine serum and 10% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
References: 22185: Jessop NW, Hay RJ. Characteristics of two rat pancreatic exocrine cell lines derived from transplantable tumors. In Vitro 16: 212, 1980.
22384: Longnecker DS, et al. AR42J细胞Transplantation of azaserine-induced carcinomas of pancreas in rats. Cancer Lett. 7: 197-202, 1979. PubMed: 509403
22884: Cockell M, et al. Identification of a cell-specific DNA-binding activity that interacts with a transcriptional activator of genes expressed in the acinar pancreas. Mol. Cell. Biol. 9: 2464-2476, 1989. PubMed: 2788241
22978: Roux E, et al. The cell-specific transcription factor PTF1 contains two different subunits that interact with the DNA. Genes Dev. 3: 1613-1624, 1989. PubMed: 2612907
23083: Seva C, et al. Lorglumide and loxiglumide inhibit gastrin-stimulated DNA synthesis in a rat tumoral acinar pancreatic cell line (AR42J). Cancer Res. 50: 5829-5833, 1990. PubMed: 2393852
23152: Rajasekaran AK, et al. Structural reorganization of the rough endoplasmic reticulum without size expansion accounts for dexamethasone-induced secretory activity in AR42J cells. J. Cell Sci. 105: 333-345, 1993. PubMed: 7691838
23222: Longnecker DS, et al. Effect of age on nodule induction by azaserine and DNA synthesis in rat pancreas. J. Natl. Cancer Inst. 58: 1769-1775, 1977. PubMed: 864754
23408: Huang Y, Hui DY. Cholesterol esterase biosynthesis in rat pancreatic AR42J cells. Post- transcriptional activation by gastric hormones. J. Biol. Chem. 266: 6720-6725, 1991. PubMed: 2016288
23412: Menniti FS, et al. Turnover of inositol polyphosphate pyrophosphates in pancreatoma cells. J. Biol. Chem. 268: 3850-3856, 1993. PubMed: 8382679
23421: Logsdon CD, et al. Glucocorticoids increase amylase mRNA levels, secretory organelles, and secretion in pancreatic acinar AR42J cells. J. Cell Biol. 100: 1200-1208, 1985. PubMed: 2579957
23552: Zhao H, et al. Regulation of intracellular Ca2+ oscillation in AR42J cells. J. Biol. Chem. 265: 20856-20862, 1990. PubMed: 1701171
23554: Zhao H, Muallem S. Inhibition of inositol 1,4,5-trisphosphate-mediated Ca2+ release by Ca2+ in cells from peripheral tissues. J. Biol. Chem. 265: 21419-21422, 1990. PubMed: 2174872
23556: Ihara H, Nakanishi S. AR42J细胞Selective inhibition of expression of the substance P receptor mRNA in pancreatic acinar AR42J cells by glucocorticoids. J. Biol. Chem. 265: 22441-22445, 1990. PubMed: 1702421
48308: Adell T, et al. Role of the basic helix-loop-helix transcription factor p48 in the differentiation phenotype of exocrine pancreas cancer cells. Cell Growth Differ. 11: 137-147, 2000. PubMed: 10768861
48309: Seva C, et al. Growth-promoting effects of glycine-extended progastrin. Science 265: 410-412, 1994. PubMed: 8023165
48311: Negre F, et al. Autocrine stimulation of AR4-2J rat pancreatic tumor cell growth by glycine-extended gastrin. Int. J. Cancer 66: 653-658, 1996. PubMed: 8647628
48312: Bertrand V, et al. Inhibition of gastrin-induced proliferation of AR4-2J cells by calcium channel antagonists. Int. J. Cancer 56: 427-432, 1994. PubMed: 7508895
57433: Mashima H, et al. Betacellulin and activin A coordinately convert amylase-secreting pancreatic AR42J cells into insulin-secreting cells. J. Clin. Invest. 97: 1647-1654, 1996. PubMed: 8601630
57434: Palgi J, et al. Transcription factor expression and hormone production in pancreatic AR42J cells. Mol. Cell. Endocrinol. 165: 41-49, 2000. PubMed: 10940482
90275: Mashima H, et al. Formation of insulin-producing cells from pancreatic acinar AR42J cells by hepatocyte growth factor. Endocrinology 137: 3969-3976, 1993. PubMed: 8756573
90276: Silver K, Yao F. ARIP cells as a model for pancreatic beta cell growth and development. Pancreas 22: 141-147, 2001. PubMed: 11249068
